Repair Replication in Escherichia coli As Measured by the Photolysis of Bromodeoxyuridine

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The Role of Nucleotide Excision Repair in Restoring Replication Following UV-Induced Damage in Escherichia coli

Following low levels of UV exposure, Escherichia coli cells deficient in nucleotide excision repair (uvrA6 mutants) recover and synthesize DNA at near wild type levels, an observation that formed the basis of the post-replication recombination repair model. In this study, we characterized the DNA synthesis that occurs following UV-irradiation in the absence of nucleotide excision repair and sho...

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Replication of the Escherichia coli chromosome.

Cell size and DNA concentration were measured in Escherichia coli K-12 ET64. This strain carries a dnaA(Ts) mutation that has been suppressed by the insertion of the F plasmid into the chrontosome. ET64 can grow in a balanced steady state of exponential growth at the restrictive temperature for its dnaA allele (39°C), in which chromosome replication is controlled by the F plasmid, and at the pe...

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ژورنال

عنوان ژورنال: Biophysical Journal

سال: 1972

ISSN: 0006-3495

DOI: 10.1016/s0006-3495(72)86094-6